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Allometric growth patterns and development in larvae and juveniles of thick-lipped grey mullet Chelon labrosus reared in mesocosm conditions ArchiMer
Ben Khemis, Ines; Gisbert, Enric; Alcaraz, Carles; Zouiten, Dora; Besbes, Raouf; Zouiten, Amina; Masmoudi, Ahmed Slaheddine; Cahu, Chantal.
Allometric growth and ontogeny were studied in thick-lipped grey mullet Chelon labrosus reared in mesocosms from 1 to 71 day post hatching (dph). Multivariate allometric analysis of morphometric growth distinguished three distinct developmental stanzas separated by two morphometric metamorphosis lengths (L-m1=4.46 +/- 0.06mm; L-m2= 28.56 +/- 1.04mm). Body mass growth also showed three distinct episodes separated by two inflections, correlated with morpho-functional changes. First episode concerned pre-flexion larvae and ended around 4.5mm-L-T (14-dph), coinciding with estimated L-m1. It was distinguished by reduced growth, but intense morphogenesis and differentiation processes. Organogenesis and allometric changes indicated that development priorities...
Tipo: Text Palavras-chave: Thick-lipped grey mullet; Chelon labrosus; Larval development; Allometric growth; Mesocosm.
Ano: 2013 URL: http://archimer.ifremer.fr/doc/00166/27742/26425.pdf
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Antimicrobial and antioxidant activities of cactus polyphenols extract on seafood preservation ArchiMer
Besbes, Nadia; Joffraud, Jean-jacques; Ben Khemis, Ines; Amri, Mohamed; Sadok, Saloua.
The present work investigated the antimicrobial and antioxidant activities of polyphenols extracted from cactus (Opuntia ficus indica) fruit-peels on sardine fillets during refrigerated storage. Biochemical, microbiological and sensorial indicators of treated sardine fillets; were studied comparatively to control lot. Microbial communities were characterized using phenotyping and molecular identification of bacterial isolates; and culture-independent method (PCR-TTGE) for fingerprinting of bacterial DNA extracted from fillets. A principal component analysis (PCA) of all the studied descriptors and variables revealed that discrimination along first principal component (PC1) was mainly correlated positively with peroxidation level and storage time but...
Tipo: Text Palavras-chave: Cactus polyphenols; PCR-TTGE; Biochemical indicators; PCA analysis; Sardine fillets; Sensorial analysis.
Ano: 2016 URL: https://archimer.ifremer.fr/doc/00368/47881/47897.pdf
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Comparison of growth, digestive system maturation and skeletal development in sea bass larvae reared in an intensive or a mesocosm system ArchiMer
Zouiten, Dora; Ben Khemis, Ines; Masmoudi, Ahmed Slaheddin; Huelvan, Christine; Cahu, Chantal.
The quality of development in intensive or mesocosm hatchery-reared Dicentrarchus labrax larvae was investigated using physiological indicators assessing ontogeny. Larvae were reared in intensive (120 L tanks) and in mesocosm systems (20 m(3) enclosures) with the same feeding sequence, excluding the wild zooplankton naturally available in mesocosms. Faster growth was recorded since early development [16 day after hatching (DAH)] in the mesocosm. Maturation of the digestive system also occurred earlier as indicated by the higher amylase secretion ratios, the intestinal maturation index (alkaline phosphatase/leucine-alanine peptidase and aminopeptidase-N/leucine-alanine peptidase ratios) and the more developed intestinal epithelium at 23 DAH. Nevertheless,...
Tipo: Text Palavras-chave: Fish larvae; Hatchery; Mesocosm; Ontogenesis; Enzyme; Skeletogenesis.
Ano: 2011 URL: http://archimer.ifremer.fr/doc/00051/16225/14064.pdf
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Differential protein expression profile in the liver of pikeperch (Sander lucioperca) larvae fed with increasing levels of phospholipids ArchiMer
Hamza, Neila; Silvestre, Frederic; Mhetli, Mohamed; Ben Khemis, Ines; Dieu, Marc; Raes, Martine; Cahu, Chantal; Kestemont, Patrick.
A comparative proteomic approach was used to assess the protein expression profile in the liver of 34 days old pikeperch larvae fed from day 10 post hatching, with three isoproteic and isolipidic formulated diets varying by their phospholipid (PL) contents (% dry diet weight): 1.4% (PL1), 4.7% (PL5) and 9.5% (PL9). Using 2D-DIGE minimal labelling of liver extracts, we were able to show 56 protein spots with a differential intensity (p<0.05) depending on the dietary PL content. Among these spots, 11 proteins were un-ambiguously identified using nanoLC-MS/MS tandem mass spectrometry. In the PL9 larvae, our results indicate that the glycolytic pathway could be down-regulated due to the under-expression of the fructose biphosphate aldolase B and the...
Tipo: Text Palavras-chave: 2D-DIGE; Metabolism; Phospholipid; Pikeperch; Proteomics.
Ano: 2010 URL: http://archimer.ifremer.fr/doc/00007/11837/8575.pdf
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